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AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions Agronomy
Andrade, Felipe Aranha de; Gonçalves, Leandro Simões Azeredo; Miglioranza, Édison; Ruas, Claudete de Fátima; Ruas, Paulo Mauricio; Takahashi, Lúcia Sadayo Assari.
The present study aimed to estimate and characterize the genetic divergence between determinate and indeterminate snap bean accessions from the Universidade Estadual de Londrina (UEL) germplasm bank based on amplified fragment length polymorphism (AFLP) markers. A total of 40 and 32 accessions with determinate and indeterminate growth habits, respectively, were characterized for this purpose. Seven combinations of primers corresponding to EcoR1 and Mse1 were tested for the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were selected. The resulting products were denatured and subjected to capillary electrophoresis. The Jaccard distance was used to estimate the genetic distances between accessions, and the Unweighted Pair...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genética e Melhoramento Phaseolus vulgaris L.; Molecular markers; Amplified Fragment Length Polymorphism; Gene bank; Genetic diversity Recursos Genéticos Phaseolus vulgaris L.; Molecular markers; Amplified fragment length polymorphism; Gene bank.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/25577
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Effects of the hydrogen potential and fungicide treatment on Pitaya seed germination Agronomy
Ortiz, Thiago Alberto; Moritz, Aline; Oliveira, Mariana Alves de; Takahashi, Lúcia Sadayo Assari.
The objective of the present study was to evaluate pitaya seed germination under different hydrogen potentials, with or without fungicide treatment. A completely randomized design was employed under a 3 x 13 x 2 factorial scheme, corresponding to three pitaya species (white pitaya, pitaya hybrid I, pitaya hybrid II), thirteen hydrogen potentials (3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0) and two fungicide conditions (presence and absence), with four replications. The percentage of germination, germination speed index and mean germination time were evaluated. The data were subjected to ANOVA, and the means were compared using the Scott-Knott test (p < 0.05). The pH influenced the germination speed index in white pitaya. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hylocereus undatus H; Costaricensis; Carboxim+thiran; Vigor; Viability; Physiological quality Tecnologia e produção de sementes.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/18088
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